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palmtracer plugin under  (MetaMorph Inc)

 
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    Structured Review

    MetaMorph Inc palmtracer plugin under
    PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using <t>PALMTracer</t> from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.
    Palmtracer Plugin Under, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/palmtracer plugin under/product/MetaMorph Inc
    Average 90 stars, based on 1 article reviews
    palmtracer plugin under - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "Imaging Minimal Bacteria at the Nanoscale: a Reliable and Versatile Process to Perform Single-Molecule Localization Microscopy in Mycoplasmas"

    Article Title: Imaging Minimal Bacteria at the Nanoscale: a Reliable and Versatile Process to Perform Single-Molecule Localization Microscopy in Mycoplasmas

    Journal: Microbiology Spectrum

    doi: 10.1128/spectrum.00645-22

    PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using PALMTracer from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.
    Figure Legend Snippet: PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using PALMTracer from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.

    Techniques Used: Imaging, Expressing, Variant Assay, Mutagenesis, Fluorescence, Derivative Assay



    Similar Products

    palmtracer plugin under  (MetaMorph Inc)
    90
    MetaMorph Inc palmtracer plugin under
    PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using <t>PALMTracer</t> from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.
    Palmtracer Plugin Under, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/palmtracer plugin under/product/MetaMorph Inc
    Average 90 stars, based on 1 article reviews
    palmtracer plugin under - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using PALMTracer from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.

    Journal: Microbiology Spectrum

    Article Title: Imaging Minimal Bacteria at the Nanoscale: a Reliable and Versatile Process to Perform Single-Molecule Localization Microscopy in Mycoplasmas

    doi: 10.1128/spectrum.00645-22

    Figure Lengend Snippet: PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using PALMTracer from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.

    Article Snippet: Based on the acquired image stacks, the localization of each individual fluorescence emitter was determined through the PALMTracer plugin under MetaMorph, and then superresolved images were reconstructed and analyzed by automatic Voronoï-based segmentation of these localizations ( , Fig. S2).

    Techniques: Imaging, Expressing, Variant Assay, Mutagenesis, Fluorescence, Derivative Assay